Encyclopaedia Britannica, 11th Edition, Volume 4, Part 3 / "Brescia" to "Bulgaria"

The preparation of the Japanese beer saké (q.v.) is of interest. The first stage consists in the preparation of Koji, which is obtained by treating steamed rice with a culture of Aspergillus oryzae. This micro-organism converts the starch into sugar. The Koji is converted into moto by adding it to a thin paste of fresh-boiled starch in a vat. Fermentation is set up and lasts for 30 to 40 days. The third stage consists in adding more rice and Koji to the moto, together with some water. A secondary fermentation, lasting from 8 to 10 days, ensues. Subsequently the whole is filtered, heated and run into casks, and is then known as saké. The interest of this process consists in the fact that a single micro-organism?a mould?is able to exercise the combined functions of saccharification and fermentation. It replaces the diastase of malted grain and also the yeast of a European brewery. Another liquid of interest is Weissbier. This, which is largely produced in Berlin (and in some respects resembles the wheat-beer produced in parts of England), is generally prepared from a mash of three parts of wheat malt and one part of barley malt. The fermentation is of a symbiotic nature, two organisms, namely a yeast and a fission fungus (the lactic acid bacillus) taking part in it. The preparation of this peculiar double ferment is assisted by the addition of a certain quantity of white wine to the yeast prior to fermentation.